Rat Anti-CD102 (CD102 Antigen, Intercellular Adhesion Molecule 2, ICAM 2, ICAM2, ICAM-2, Ly60) (Low Endotoxin)
Murine CD102 is a glycosylated cell surface protein that is broadly expressed on most leukocytes and is constitutively expressed on endothelial cells. CD102 interacts with the CD11a/CD18 integrin LFA-1. Reports suggest that CD102 may play a role in lymphocyte re-circulation and T-cell activation. Reported to inhibit interactions between CD102 and LFA-1.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), Functional Activity and Immunoprecipitation . Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50-1:200 Use 10ul to label 10e6 cells in 100ul. Optimal dilution determined by the researcher
Hybridoma
Spleen cells from immunized Lewis rats were fused with cells of the Mouse P3X63Ag8.653 myeloma cell line..
Recommended Secondary Reagents
F(ab')2 Goat anti rat IgG:FITC (mouse adsorbed) F(ab')2 Goat anti rat IgG:RPE (mouse adsorbed)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
COS cells transfected with murine ICAM-2.
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant. Endotoxin: <0.01EU/ug
Specificity
Recognizes mouse intercellular adhesion molecule-2 (ICAM-2).
