Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Flow Cytometry: 1:50-1:100 Use 10ul of the suggested working dilution to label 5x10e5 cells in 100ul. Immunohistochemistry (frozen): 1:50-1:100 Optimal working dilutions to be determined by researcher.

Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Intestine.

I1904-65W IgG, F(ab’)2 (HRP) Pab Rb xMo I1904-66C IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb xMo

I1904-83P1: IgG2a Murine Negative Control

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human Intestinal lymphocyte.

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.

Purified by Protein A affinity chromatography.

Recognizes the human CD103 cell surface antigen, also known as the alpha-E integrin. This reacts with various subsets of human intra-epithelial lymphocytes:- 40% of lamina propria T-cells; 30% of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognizes less than 0.1% bone marrow cells and less than 0.7% PBL. Species Crossreactivity: Rhesus monkey, monkey and Cynomolgus monkey.