Mouse Anti-CD103 (PE)
CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. This antibody is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins.1 The property of costimulation is not unique to the mouse since the combination of anti-human alphaIEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.
Applications
Suitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Recommended Negative Controls
I1904-79D: IgG1 Murine Negative Control (PE)
Storage and Stability
Lyophilized powder may be stored at 4°C. Reconstitute by adding sterile buffer or ddH2O. Aliquot and store at 4°C. DO NOT FREEZE! Reconstituted product is stable for 12 months. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Hairy cell leukaemia cells.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml sterile ddH2O.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the human CD103 cell surface antigen, a glycoprotein expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes. It has recently been shown to be identical to the alphaE integrin.
