Technical Data

C2446-87
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG1,k
Clone Number
2Q676 (M/K-2)
Grade
Purified
Applications
FC IHC IP WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rat Anti-CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (Azide Free, Low Endotoxin)
Applications
Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. May be used for the following: Blockage of lymphocyte adhesion in Whitlock-Witte long-term bone marrow cultures (1, 2), Inhibition of lymphopoiesis in Whitlock-Witte cultures (1, 2) Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
BALB/3T3 and +/+2.4 cells
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Purity
Purified
Specificity
Recognizes mouse CD106. Immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100kD under reducing conditions and 92kD under non-reducing conditions.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Miyake, K., I.L. Weissman, J.S. Greenberger and P. W. Kincade. 1991. J. Exp. Med. 173:599.|2. Miyake, K., et al.. 1991. J. Cell. Biol. 114(3):557.|3. Schlegel, P.G., et al. 1995. J. Immunol. 155:3856.|4. Bergese, S., et al. 1995. Am J. Phathol. 146:989.|5. Orosz, C.G., et al. 1993. Transplantation 56:453.
USBio References
No references available
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