The c-kit proto-oncogene is the cellular homologue of the feline sarcoma v-kit oncogene. It encodes a type III transmembrane receptor tyrosine kinase also known as CD117 or stem cell factor receptor (SCFR). c-Kit signaling is activated when a ligand known as steel factor or stem cell factor (SCF) binds to the receptor. Interactions between specific phosphotyrosine residues of activated receptor tyrosine kinases (RTKs) and Src homology 2 (SH2) domains define cellular growth and differentiation signals. It has been reported that SCF stimulates the association of the p85 subunit of PI3K with the phosphorylated tyrosine-721 residue within wild type kit. Subsequently, PI3K contributes to the activation of Akt and JNKs. c-Kit is necessary for the development of hematopoietic progenitors, mast cells, germ cells, melanocytes, and the interstitial cells of Cajal (ICC). Oncogenic c-kit mutations have been implicated in neoplasms arising from these stem cell lineages, such as gastrointestinal stromal tumor (GIST). The mature, phosphorylated form of c-Kit is a glycosylated protein with a molecular weight of ~145kD, while the immature form is ~125kD.

Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Western Blot: 1-3ug/ml Immunohistochemistry: 5-10ug/ml Note: Prior to immunohistochemistry with paraffin-embedded tissue sections, heat induced epitope retrieval with EDTA, pH 8.0. Optimal dilutions to be determined by the researcher.

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Synthetic peptide derived from an internal sequence of the human c-Kit protein

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.

Purified by epitope affinity chromatography.

Reacts with the human c-Kit (CD117 or stem cell factor receptor (SCFR)) oncoprotein. On Western blots, this antibody identifies a ~145kD band representing the mature (phosphorylated, glycosylated) form of c-Kit, a ~125kD band for the immature form, and a ~95kD band representing the soluble form. Reactivity has been confirmed with human GIST-882 and EWS-502 cell lysates.