C2548-80H-APC
Clone Type
MonoclonalHost
RatSource
MouseConjugate
APCIsotype
IgG1Clone Number
6A373 (OX114)Grade
Affinity PurifiedApplications
FC IHC WBCrossreactivity
MoShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Rat Anti-CD147 (Extracellular Matrix Metalloproteinase Inducer, Basigin, Neurothelin, 5A11 antigen, 5F7, Blood-brain barrier HT7 antigen, Collagenase stimulatory factor, Emmprin, Leukocyte activation antigen M6, M6, OK, OK blood group antigen, TCSF, Tumor cell derived collagenase stimulatory factor) (APC)
CD147 (50-60kD) has a wide tissue distribution on hematopoietic and non-hematopoietic cells including leukocytes, red blood cells, platelets and endothelial cells. As a potential adhesion molecule, CD147 may be involved in embryonic blood-brain barrier development and in integrin-mediated adhesion in brain endothelia. The reported functions of CD147 are diverse and include involvement in T-cell activation, cell adhesion and induction of matrix metalloproteinases.
Applications
Suitable for use in Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemstry: Frozen sections Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-76H: Rat IgG1 Negative Control
Recommended Secondary Reagents
I1904-47Y: IgG, Fc (HRP) Pab Gt xRt I1904-47T: IgG, H&L (HRP) Pab Gt xRt I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRt I1904-47H: IgG, H&L (HRP) Pab Gt xRt I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab Gt xRt
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Fusion protein mouse CD147dO-2rCD4d3 +4.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse CD147 (also known as basigin). Recognizes both isoforms of CD147, the common form containing 2 Ig-like domains and the rare form containing 3 Ig-like domains.
