Mouse Anti-CD147 (Basigin, 5F7, Collagenase Stimulatory Factor, Extracellular Matrix Metalloproteinase Inducer, EMMPRIN, TCSF, Leukocyte Activation Antigen M6, OK Blood Group Antigen, Tumor Cell-derived Collagenase Stimulatory Factor, UNQ6505/PRO21383)
CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Flow Cytometry: ≤0.5ug/10e6 cells in 100ul volume or 100ul whole blood Immunohistochemistry (Frozen/FFPE): 0.5-10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by affinity chromatography
Specificity
Recognizes human CD147. Species Crossreactivity: chimpanzee, baboon and Capuchin monkey
