Technical Data

C2548-84T
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
TRAP1.3.6
Grade
Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD154 (CD40 Ligand, CD40-L, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen GP39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNF Ligand Superfamily Member 5, TNFSF5)
CD40 Antigen Ligand, Hyper IgM Syndrome, T-B cell Activating Molecule

Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. Interaction of CD154 and CD40 is essential for isotype switching in B cells. Known genetic defects that alter this interaction lead to impaired immune system function. CD154 has been shown to be hyperexpressed by B and T cells in SLE patients.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:10-1:50 10ul labels 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse myeloma cell line transfected with human CD40L (CD154).
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes human CD154.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hermann, P. et al. (1993). Expression of a 32kD ligand for the CD40 antigen on activated human T lymphocytes. Eur. J. Immunol. 23:961-964. |2. Lane, P. et al. (1992). Activated human T cells express a ligand for the human B cell associated antigen CD40 which participates in T cell-dependent activation of B lymphocytes. Eur. J. Immunol. 22:2573-2578. |3. Kroszek, R.A. et al. (1994). Defective expression of CD40 ligand on T cells causes "X-linked Immunodeficiency with hyper-IgM (HIGML) Immunol. Rev. 138:39-59.
USBio References
No references available
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