Mouse Anti-CD154, soluble (CD40 Ligand, CD40-L, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen GP39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNF Ligand Superfamily Member 5, TNFSF5) (MaxLight 490)
CD40 Antigen Ligand, Hyper IgM Syndrome, T-B cell Activating Molecule
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
CD154 is a member of the TNF family of molecules. It binds to CD40 and acts as a co-stimulatory molecule by activating antigen presenting cells, such as B cells. While CD154 is mostly found expressed on activated T cells.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
Spleen cells from immunized Balb/c mice were fused with cells of the myeloma Sp2/0 cell line.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant human soluble CD154 conjugated to BSA.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD154, a 32kD glycoprotein also known as CD40 ligand.
