Mouse Anti-CD178 (Apoptosis Antigen Ligand, APT1LG1, APTL, CD95 Ligand, CD95L, CD95-L, FAS Antigen Ligand, Fas Ligand, FASL, FASLG, Tumor Necrosis Factor Ligand Superfamily Member 6, TNFSF6)
TNF Superfamily Member 6
CD178 cell surface antigen is a 40kD glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.
Applications
Suitable for use in Flow Cytometry, ELISA and Immunoprecipitation. Does not work with Western Blot. Other applications have not been tested.
Recommended Dilution
Flow Cytometry: 1:25-1:50. Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Hybridoma
Spleen cells from immunized BALB/c mice were fused with cells of the P3U1 myeloma cell line.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD178.
