Technical Data

C2548-93C
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2a
Clone Number
RP/14
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-CD180 (RP105, Radioprotective 105)

Radioprotective 105 (RP105, CD180) is a type 1 transmembrane protein of 105kD with extracellular leucine-riche repeats (LLRs) and a short cytoplasmic tail. RP105, assigned as CD180, is similar to Drosophila Toll in the extracellular LLRs. Radioprotective 105 (RP105, CD180) expression is largely restricted to immune cells including CD19+ B cells and macrophages. Histological studies showed that RP105 is expressed mainly on mature B cells in mantle zones. Antibody-mediated cross-linking of RP105 induces resistance against irradiation-induced apoptosis, B-cell proliferation, and up-regulation of a costimulatory molecule B7.2, revealing RP105 as a potent regulator of B-cell activation. RP105 (CD180) has an important role in B-cell activation by LPS. It is important to note that RP105 associates with MD1, which is in this context important for RP105 with respect to B-cell surface expression and LPS recognition and signalling. MD-1 seems to be requisite for efficient expression of RP105.

Applications
Sutiable for use in Flow Cytometry and B cell activation in functional studies. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse Radioprotective 105.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Miyake, K., et al; Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression. J Immunol 1998, 161: 1348. 2. Nagai, Y et al; Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Blood 2002, 99: 1699.
USBio References
No references available
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