C2548-97D8-ML490
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™490Isotype
IgG1Clone Number
3H1998 (HLy9.1.25)Grade
Affinity PurifiedApplications
FC IPCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-CD229 (Cell Surface Molecule Ly9, hLy9, Lymphocyte Antigen 9, Ly 9, Ly9, Ly-9, mLY9, SLAMF3, T Lymphocyte Surface Antigen Ly9 Precursor) (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.
Applications
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Hybridoma
NS-1 myeloma cells with spleen cells from Balb/c mice.
Recommended Secondary Antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Mouse pre-B cell line 300.19 transfected with CD229 cDNA.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD229.
