Mouse Anti-CD236 (Glycophorin C, GpC) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Applications
Immunohistochemistry (frozen) Immunoprecipitation Western Blot Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP
Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the myeloma cell line.
Recommended Negative Controls: Mouse IgG1 Negative Control
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to amino acids in the cytoplasmic domain of Glycophorin C, GpC. Sequence is aa112-128 (DPALQDAGDSSRKEYFI)
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Specificity
Recognizes a 17 amino acid residue within the cytoplasmic domain of glycophorin C (GPC), which has recently been designated CD236.
