Technical Data

C2549-18A2
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG2a,k
Clone Number
7H150
Grade
Affinity Purified
Applications
FC FLISA
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD270 (ATAR, Herpes Virus Entry Mediator A, Herpes virus Entry Mediator A, HveA, HVEM, LIGHTR, Tumor Necrosis Factor Receptor Superfamily Member 14, TNFRSF14, Tumor Necrosis Factor Receptor-like 2, TR2, UNQ329/PRO509) (FITC)

Human CD270 (Herpes Virus Entry Mediator, TNFRSF14, ATAR, LIGHTR, TR2) is a 36kD type I membrane protein that has been found on a broad range of lymphoid tissues. Its ligands include the TNF Ligand family members Lt and LIGHT, and the CD28/Ig Superfamily member BTLA (2).

Applications
Suitable for use in FLISA and Flow Cytometry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Immunogen
Recombinant corresponding to human CD270-muIg fusion protein
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD270 (HVEM). Reactive with recombinant CD270 in EIA, and binds to CD270 present on human U-937 cell surface in Flow Cytometry.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hsu H, et al. (1997) J Biol Chem 272:13471, 2. Gonzalez LC, et al. (2005) PNAS 102(4): 1116-1121.
USBio References
No references available
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