Mouse Anti-CD274 (CD274 Molecule, B7 Homolog 1, B7H1, B7-H1, B7-H, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1)
MGC142294, MGC142296
CD274 (B7-H1, PD-L1, Programmed Death Ligand) is a member of the B7 family and is expressed on a variety of tissues including lymphoid cells. It plays an important role in regulation of T cell activation, and is involved in progression of cancer, arthritis and HIV infection. CD274 binding to its receptor CD279 (PD-1) on activated T cells can decrease proliferation. Conversely, ligation of CD279 on primed T cells can stimulate IL-10 production. High levels of CD274 present in Renal cell carcinoma is associated with poor prognosis. Tumor expressed CD274 can increase apoptosis of tumor specific T cells resulting in better tumor cell survival. Gamma Interferon and PHA can up regulate CD274 expression on T cells.
Applications
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 5ug/ml (80ul) Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol
1. Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5ug/ml PHA. 2. Five x10e5 cells/tube were incubated 45 minutes on ice with 80ul of C2549-22P1 at 5ug/ml. 3. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed twice, fixed and analyzed by FACS using a lymphoid gate. 4. Cells stained positive with a mean shift of 0.33 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. 5. Binding was blocked when reagent was pre incubated with a 10-fold excess of C2549-22R.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human CD274.
Form
Supplied as a liquid in PBS. Preservative free.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD274 (B7-H1). Binds to recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.
