Goat Anti-CDCP1, isoform 1, CT (CUB domain containing protein 1, CD318, Membrane glycoprotein gp140, SIMA135, Subtractive immunization M plus HEp3-associated 135kD protein, Transmembrane and associated with src kinases, TRASK, UNQ2486/PRO5773)
CDCP1(CUB-domain containing protein 1; also CD318 and SIMA135) is a novel, 135kD cell surface glycoprotein that is found on tumor, stem cells, keratinocytes and colonic epithelial cells. It is reported that this protein is overexpressed in colon and lung cancers. It is a type I transmembrane (TM) protein that is involved with cell adhesion. Mouse CDCP1 is synthesized as an 833 amino acid (aa) precursor. It contains an extracellular region with three CUB domains (aa30-667) and a phosphotyrosine site at Tyr731. When unligated, CDCP1 can be proteolytically cleaved between aa270-300. This generates an 80kD TM protein that may be missing the N-terminal CUB domain (aa 221-350). Over aa25-667, mouse CDCP1 is 83% and 92% aa identical to human and rat CDCP1, respectively.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-PLLNTQEPMEPAE, from the C Terminus of human CDCP1 (NP_073753.3).
Form
Supplied as a liquid in Tris saline, pH7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CDCP1.
