C2572-29
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E FC IP WBCrossreactivity
Hu Mo RtShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Rabbit Anti-cdk2, phosphorylated (Thr160) (Cyclin-dependent Kinase 2)
Cyclin-dependent kinase 2, p33cdk2, is an important component of the cell cycle machinery. Like p34cdc2, its kinase activity is regulated by association with a cyclin subunit, by its phosphorylation state and by association with a cdk inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15. Inhibition of cdk2-cyclin complexes can also be attributed to association with p27Kip1 and p21Waf1/Cip1. Activation of cdk2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK, a complex of cdk7 and cyclin H. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2.
Applications
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
ELISA (Peptide): 1:2000 Flow Cytometry: 1:25 Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:150 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr160 of human cdk2.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of human CDK2 only when phosphorylated at threonine 160. The antibody weakly cross-reacts with cdc2 phosphorylated at Thr161. Does not cross-react with other phosphorylated cyclin dependent kinases. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Morgan, D.O., Nature 374: 131-134 (1995). 2. Poon, R.Y., et al., J. Biol. Chem. 271: 13,283-13,291 (1996). 3. Gu, Y., et al., EMBO J. 11: 3995-4005 (1992). 4. Fesquet, D., et al., EMBO J. 12: 3111-3121 (1993). 5. Morgan, D.O., Annu. Rev. Cell Dev. Biol. 13: 261-291 (1997). 6. Chiariello, M., et al., Biochem. J. 349: 869-876 (2000). 7. Lents, N.H., et al., J. Biol. Chem. 277: 47,469-47,475 (2002).USBio References
No references available