Mouse Anti-CEACAM-4 (Carcinoembryonic Antigen Related Cell Adhesion Molecule 4)
Carcinoembryonic antigen cell adhesion molecule 4 (CEACAM-4), also known as CGM7 and W236, is a 40kD transmembrane glycoprotein member of the CEACAM family of proteins. Its 209aa extracellular domain contains one Ig-like V-type domain. CEACAM-4 is expressed on granulocytes as well as on B and T cell lymphoblastic leukemias. Mouse and rat orthologs of CEACAM-4 have not been described.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Direct ELISA: 0.5-1ug/ml Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher.
Hybridoma
Myeloma cells with B cells from Balb/c mice.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to human CEACAM-4 expressed in E. coli (O75871).
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CEACAM-4.
