Rabbit Anti-Chloramphenicol Acetyltransferase (CAT)
E. coli
Chloramphenicol Acetyltransferase (CAT) is suitable for use in cell extract assays. CAT, encoded by Tn9, catalyzes the transfer of an acetyl group from acetyl-CoA to the 3’-hydroxy position of chloramphenicol.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1:500 Western Blot: 1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Chloramphenicol Acetyltransferase (CAT)
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide
Specificity
Specific for CAT fusion proteins that contain, from the N-terminus, one third or more of the CAT protein.
