Technical Data

C5042-25K
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IC IF IHC WB
Crossreactivity
Hu Mo Rt
Accession #
NP_795344.1
Gene ID
887, 12426, 25706
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-CCKBR (Gastrin/Cholecystokinin Type B Receptor, CCK-B Receptor, CCK-BR, Cholecystokinin-2 Receptor, CCK2-R, CCKRB)

CCKB receptor, also celled gastrin receptor, is a Cholecystokinin Receptor. CCKB receptor binds both sulfated and non-sulfated cholecystokinin analogs. This receptor regulates anxiety, arousal, opiate-induced analgesia, and gastric-acid secretion. CCKB receptor has been reported in brain, colon, pancreas, stomach, thyroid, and various cancers. ESTs have been isolated from normal eye libraries and libraries derived from cancers of the brain, embryo, germ cell, lung, and placenta.

Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA (Peptide): 1:16000 Western Blot: 1-3ug/ml Immunohistochemistry (Paraffin): 5ug/ml Uterus 6ug/ml pancreas Immunofluorescence (IC): See reference #2 and #3 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-FDGDSDSDSQSRVRNQ, of human CCKBR, at the internal region of the protein. Species Sequence Homology: bovine
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CCKBR. Species Crossreactivity: rat and mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. El-Kouhen K, Morisset J. Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells. Int J Endocrinol. 2009;2009:875167. 2. Berna MJ, Seiz O, Nast JF, Benten D, Bläker M, Koch J, Lohse AW, Pace A. CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce collagen production. J Biol Chem. 2010 Dec 10;285(50):38905-14. 3. El-Kouhen K, Morisset J. Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line. Pancreas. 2010 Mar;39(2):127-34.
USBio References
No references available
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