Mouse Anti-Chorionic Gonadotropin, Human, beta (hCGb)
Human Chorionic Gonadotropin (HCG) is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the a-and b-subunits. The a-subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP), and HCG but negative for EMA and AFP is probably a choriocarcinoma.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Affinity Constant
2.0x10e11 L/mole
Matched Pair
Capture: C5069-67H Detection: C5069-67B
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human Chorionic Gonadotropin isolated from human pregnancy urine.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography. > 90% by SDS-PAGE.
Specificity
Recognizes human intact-hCG and its beta subunit. Does not react with hCG alpha subunit.
