Mouse Anti-Chorionic Gonadotropin, Human, beta (hCGb) (MaxLight 650)
MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Human Chorionic Gonadotropin (HCG) is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the a-and b-subunits. The a-subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP), and HCG but negative for EMA and AFP is probably a choriocarcinoma.
Applications
Suitable for use in very sensitive and specific gold or latex based single step assay. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Affinity Constant
~8.0x10e9L/mole
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Highly purified Human Chorionic Gonadotropin, beta
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by chromatography.
Specificity
Recognizes Human Chorionic Gonadotropin, beta |Crossreactivity: hCG: <90%; hTSH, hLH, hFSH: ≤1%.
