C5069-94C
Clone Type
MonoclonalHost
RabbitSource
HumanIsotype
IgGClone Number
11C1020(D77D5)Grade
PurifiedApplications
IC IF IP WBCrossreactivity
Hu MkGene ID
10036Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Chromatin Assembly Factor 1 (CAF1, CAF-1, CAF, Chromatin Assembly Factor 1 Subunit A, CAF-1 Subunit A, CHAF1A, CAF1B, Chromatin Assembly Factor I p150 Subunit, CAF-I 150kD Subunit, CAF1P150, CAF-I p150, hp150, MGC71229, P150)
Chromatin Assembly Factor 1, Subunit A (p150)
Chromatin assembly factor 1 (CAF-1) is a histone H3/H4 chaperone complex that functions in de novo assembly of nucleosomes during DNA replication and nucleotide excision repair (1). Nucleosome assembly is a two-step process, involving initial deposition of a histone H3/H4 tetramer onto DNA, followed by the deposition of a pair of histone H2A/H2B dimers (1). CAF-1 interacts with PCNA and localizes to DNA replication and DNA repair foci, where it functions to assemble newly synthesized histone H3/H4 tetramers onto replicating DNA (2-6). Assembly of histone H2A/H2B dimers requires additional assembly factors. The CAF-1 complex consists of three proteins: CHAF1A (p150), CHAF1B (p60) and RBAP48 (p48 or RBBP4). CHAF1A and CHAF1B proteins are specific for the CAF-1 complex, while RBAP48 is a component of multiple chromatin modifying complexes (1). CHAF1A and CHAF1B expression levels correlate with cellular proliferation and both proteins are significantly down-regulated in quiescent cells (7).
Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Immunofluorescence (IF-IC): 1:200 Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Pro303 of human CHAF1A.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity
Recognizes endogenous levels of total human CHAF1A. Species Crossreactivity: monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
(1) Adams, C.R. and Kamakaka, R.T. (1999) Curr Opin Genet Dev 9, 185-90. (2) Smith, S. and Stillman, B. (1989) Cell 58, 15-25. (3) Smith, S. and Stillman, B. (1991) EMBO J 10, 971-80. (4) Shibahara, K. and Stillman, B. (1999) Cell 96, 575-85. (5) Moggs, J.G. et al. (2000) Mol Cell Biol 20, 1206-18. (6) Gaillard, P.H. et al. (1996) Cell 86, 887-96. (7) Polo, S.E. et al. (2004) Cancer Res 64, 2371-81.USBio References
No references available