C5815-10B
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E FC IC IHC WBCrossreactivity
Hu Mo RtShipping Temp
Blue IceStorage Temp
4°C (-20°C Glycerol)Notes
Preservative Free
Rabbit Anti-c-Jun, phosphorylated (Ser63) (BSA, Azide and Glycerol Free)
c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c- Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV irradiation, stimulate phosphorylation of c-Jun at Ser63/73 and activate c- Jun-dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stressinduced signaling pathways. The MAP kinase homologue SAPK/JNK binds to the N-terminal region of c- Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).
Applications
Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:100 Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50 Immunocytochemistry (ABC): 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic phosphopeptide corresponding to residues around Ser63 of human c-Jun (KLH coupled).
Form
Supplied as liquid in PBS. No preservative added. No stabilizing proteins added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Detects endogenous levels of human c-Jun only when phosphorylated at Ser63. Does not recognize the phosphorylated forms of JunD or JunB. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Binetruy, B., et al., Nature 351: 122–127 (1991). 2. Smeal, T., et al., Nature 354: 494–496 (1991). 3. Derijard, B., et al., Cell 76: 1025–1037 (1994). 4. Kyriakis, J.M., et al., Nature 369: 156–160 (1994). 5. de Ruiter, N.D., et al., Mol. Cell. Biol. 20: 8480–8488 (2000). 6. Garcia, M., et al., J. Neurosci. 22: 2174–2184 (2002). 7. Gonzalez, M.V., et al., J. Cell Biol. 150: 1199–1208 (2000). 8. Kleemann, R., et al., Nature 408: 211–216 (2000). 9. Lee, S.A., et al., J. Biol. Chem. 276: 11,783–11,790 (2001). 10. Li, B., et al., EMBO J. 21: 324–333 (2002). 11. Linseman, D.A., et al., J. Biol. Chem. 276: 39,123–39,131 (2001). 12. Zhang, S.Q., Immunity 12: 301–311 (2000).USBio References
No references available