Technical Data

C5831-03D
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG2b
Clone Number
10K159
Grade
Affinity Purified
Applications
IF WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CLASP2 (CLIP-associating Protein 2, Cytoplasmic Linker-associated Protein 2, Protein Orbit Homolog 2, hOrbit2, KIAA0627)

CLASP2 is microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.

Applications
Suitable for use in Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilution
Western Blot: 1:1,000 Immunofluorescence: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Alpha-CL2-N-Term and GST fusion protein.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes rat CLASP2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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