C5837-05E
Clone Type
PolyclonalHost
RabbitSource
RatIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
RtShipping Temp
Blue IceStorage Temp
4°C (-20°C Glycerol)Notes
Preservative Free
Rabbit Anti-CLC2 (Clcn2, Chloride Channel)
Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ions. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000aa) are predicted to contain 10 (possibly 12) transmembrane domains. Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues. Rat CLC-2 is 887aa (or 907aa) membrane protein (human CLC-2 898aa) (1). An alternatively spliced short form (491aa) has also been found. CLC-2 is ubiquitously expressed. Defects in CLC1 (CLCN1) are the cause of autosomal recessive generalized myotonia (Becker's disease) (RGM) and autosomal dominant myotonia congenita (Thomsen's disease; MC) which are characterized by skeletal muscle stiffness (delayed relaxation) that is a result of muscle membrane hyperexcitability.
Applications
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Immunohistochemistry: 1-20ug/ml in paraformaldehyde fixed sections of tissues. Western Blot: 1-10ug/ml using ECL. An antibody made to the C-terminal region has detected multiple CLC-2 bands between 80-90kD bands in various tissues (7,8). Optimal dilutions to be determined by the researcher.
Control Peptide
C5837-05H
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide consisting of 22aa near the C-terminus of rat CLC-2 (KLH). Species Sequence Homology: Mouse: 100%; Porcine, rabbit: 95%; Human: 90%.
Form
Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat Chloride Channel 2. No significant sequence homology is detected with other CLCs or other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Chu, S., et al., Nucl. Acid. Res. 25: 4153 (1997). 2. Chu, S., et al., Nucl. Acids Res. 24: 3453 (1996). 3. Cid, L.P., et al., Hum. Mol. Genet. 4: 407 (1995). 4. Thiemann, A., et al., Nature 356: 57-60 (1992). 5. Murray, C.B., et al., Am. J. Respir. Cell. Mol. Biol. 12: 597 (1995). 6. Murray, C.B., et al., AJP 271: L829 (1996). Citations: 1. Hori, K., FEBS Lett. 575: 105-108 (2004). (WB, IHC) 2. Gustincich, S., Proc. Natl. Acad. Sci. 101: 5069-5074 (2004). (IHC)USBio References
No references available