Rabbit Anti-CLC7 (Clcn7, Chloride Channel) (Not for Export EU)
Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000 aa) are predicted to contain 10 (possibly 12) transmembrane domains. Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues.
Rat CLC-7 is 802 aa membrane protein (mouse 803 aa, human 805 aa) (1). CLC-7 has a calculated MW of approx. 90kD. CLC-7 is ~45% identical with CLC-6. CLC-7 is expressed in brain, tested, muscle and kidney (1).
Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000-50,000 Western Blot: 1:1000-5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 17aa Peptide sequence near the N-terminus of human CLC-7 (coupled to KLH).
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 40% glycerol.
Specificity
Recognizes human CLC7. No significant sequence homology is detected with other CLCs or other proteins. Species sequence homology: mouse and rat 93%.
