C5838-16-PE
Clone Type
MonoclonalHost
MouseSource
MouseConjugate
PEIsotype
IgG2bClone Number
3G95Grade
Affinity PurifiedApplications
IF IHC IP WBCrossreactivity
Ca Hu Mo RtShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-Claudin 2 (CLDN2) (PE)
Tight Junctions (TJ) are specialized membrane domains that seal the intercellular spaces in epithelia and endothelia and thus contribute to the permeability barrier between lumenal and interstitial compartments. Tight junctions not only separate distinct physiological compartments, but they also confer selectivity to the transepithelial flux of molecules and ions through the intercellular spaces between the epithelial cells, the so-called paracellular pathway. In addition, tight junctions limit the diffusion of lipids between the apical and basolateral plasma membrane domain. Claudin and Occludin, the two types of four-transmembrane domain proteins, are the major integral membrane components of the mammalian tight junction. So far, 20 distinct claudins have been identified. Mouse Claudin-2 was discovered in 1998 as a 230aa integral membrane protein localizing at tight junctions with an approximate molecular weight of 22.9kD. Claudin-1 and Claudin-2 are structurally related, and are in fact 38% identical at the amino acid sequence level. The expression of Claudin-2 is restricted to the liver and kidney, with small amounts found in the brain.
Applications
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: Reactivity has been confirmed with human, mouse, rat and dog liver and kidney homogenates, as well MDCK and Caco-2 cell lysates. A non-specific band at 27kD is observed in some lysates; in Caco-2 cells this band appears stronger than the correct band at 22 kDa. Immunhistochemistry (Paraffin): Heat-induced epitope retrieval (HIER) with EDTA buffer, pH 8.0, is required prior to staining. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to a 26 amino acid sequence at the C-terminus of the mouse Claudin-2 protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes mouse Claudin-2. Species Crossreactivity: human, canine and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Anderson JM, Curr. Biol. 6:382-384 (1996). 2. Anderson JM and Van Itallie CM, Am J. Physiol., 269:G467-475 (1995). 3. Howarth AG and Stevenson BR, Adv. Struc. Biol. 4:25-39 (1996). 4. Stevenson BR and Keon BH, Annu.Rev.Cell Dev. Biol. 14:89-109 (1998). 5. Tsukita S, et al., Cell Struct. Funct. 21:381-385 (1996). 6. Yap AS et al., H, Membr. Biol. 163:159-167 (1998). 7. Saitou M, et al., J. Cell. Biol. 141:397-408 (1998). 8. Furuse M, et al., J. Cell Biol. 143:391-401 (1998). 9. Tsukita S and Furuse M, Genes Cells 3:569-573 (1998). 10. Morita K, et al., Proc Natl Acad Sci USA 96:511-516 (1999). 11. Van Itallie CM, Rahner C, Anderson JM. J Clin Invest 107(10): 1319-1327 (2001). 12. Furuse M, et al., J Cell Biol 141(7): 1539-1550, (1998). 13. Lippoldt A, et al., Neuroreport 11(7): 1427-1431 (2000).USBio References
No references available