Claudin 4 is a multipass membrane protein in the claudin family of tight junction proteins. Claudin 4 is a 209 amino acid molecule with four transmembrane segments. It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development. It is also upregulated in several carcinomas. Phosphorylation of claudin 4 regulates paracellular epithelial permeability. Human claudin 4 shares 83% aa sequence identity with mouse and rat claudin 4.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 25ug/10e6 cells. Validated for use with PC-3 human prostate cancer cell line. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Met1-Val209 from human Claudin 4 expressed in NS0 cells.|Species Sequence Homology: mouse and rat, 83%
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human Claudin 4. Recognizes an epitope in the first extracellular loop (between aa 29-81). Stains human Claudin 4 transfectants but not irrelevant transfectants.
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