Technical Data

C5838-91
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
IF IHC WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Claudin 18 (CLDN18)

Claudin-18 is a tight junction protein expressed as lung- and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene.1 The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor.1 A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human.1 Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein.1 Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells.1 These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach.1 Claudin-18 expression has also been reported in the inner ear.2

Applications
Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry Other applications not tested.
Recommended Dilutions
Immunohistochemistry (paraffin): 1:10-1:100 Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher.
Positive Control
Mouse lung and stomach homogenates
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide derived from the C-terminal region of the human Claudin-18 protein, which differs from mouse by 4 conservative amino acid replacements and one non-conservative amino acid replacement
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes mouse Claudin-18 protein at ~29kD. |Species crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Niimi T, et al. Mol Cell Biol 21:7380-7390, 2001. 2. Kitajiri SI, et al. Hear Res 187:25-34, 2004.
USBio References
No references available
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