Mouse Anti-CLEC4E (C-type lectin domain family 4, member E)
CLEC4E is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. CLEC4E expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6,6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB). Recently it was reported that CLEC4E is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor gamma-chain (FcR-gamma) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:50. Incubated for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Immunofluorescence (IF-IC): 1:500 Optimal dilutions to be determined by the researcher.
Hybridoma
Mouse F0 myeloma cells with spleen cells from BALB/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant corresponding to aa41-219 from human CLEC4E expressed in E. coli (NP_055173).
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography. (≥95% SDS-PAGE)
Specificity
Recognizes human CLEC4E.
