Technical Data

C7506-21
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Ca Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Cofilin, phosphorylated (Ser3)

Cofilin is a 20kD actin-binding protein and a key regulator of actin dynamics involved in regulating lamellipodia formation, neurite extension, chemotaxis and tumor cell migration. Phosphorylation of cofilin by LIM kinase (LIMK1) on serine 3 mediates Rho-ROCK and Rac-PAK signaling pathways in regulating actin reorganization by inactivating cofilin and blocking its ability to promote actin-filament disassembly.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control
MDCK cells treated with staurosporine; Jurkat cells treated with hydrogen peroxide.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide derived from a region of human cofilin that contains serine 3. Species sequence homology: mouse and rat.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 50% glycerol, 1.0 mg/mL BSA (IgG, protease free), 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human and canine cofilin phosphorylated at Ser3. Species sequence homology: mouse and rat (100%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Nishita, M., et al. (2003) Phosphoinositide 3-kinase-mediated activation of cofilin phosphatase slingshot and its role for insulin-induced membrane protrusion. J. Biol. Chem. Epub ahead of print. 2. Endo, L., et al. (2003) Control of growth cone motility and morphology by LIM kinase and Slingshot via phosphorylation and dephosphorylation of cofilin. J. Neurosci. 23(7):2527-2537. 3. Sarmiere, P.D. and J.R. Bamburg (2003) Regulation of the neuronal actin cytoskeleton by ADF/cofilin. J. Neurobiol. 58(1):103-117. 4. Keezer, S.M., et al. (2003) Angiogenesis inhibitors target the endothelial cell cytoskeleton through altered regulation of heat shock protein 27 and cofilin. Cancer Res. 63(19):6405-6412. 5. Yang, N., et al. (1998) Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization. Nature 393(6687):809-812. 6. Moryama, K., et al. (1996) Phosphorylation of Ser-3 of cofilin regulates its essential function on actin. Genes Cells 1(1):73-86.
USBio References
No references available
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