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C7506-25E Rabbit Anti-Cofilin, phosphorylated (Ser3) (Cofilin-1, 18kD Phosphoprotein, p18, Cofilin, Non-muscle Isoform, CFL1, CFL)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:500-1:1000 Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phospho-peptide corresponding to the phosphorylation site of serine 3 (M-A-SP-G-V) from human Cofilin.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of human Cofilin only when phosphorylated at serine 3. Species Crossreactivity: mouse, rat
References
1. Kobayashi M., et al. (2006) EMBO J 25(4):713-26. 2. Wany Y, et al. (2005) Biol Chem 280(13):12683-9. 3. Smith-Beckerman DM., et al. (2005) Mol Cell Proteomics 156-68.
USBio References
No references available
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