Rabbit Anti-Collagen Type II
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Applications
Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1:5000-1:50,000 Immunohistochemistry: 1:50-1:400. Antigen retrieval with sodium citrate buffer, pH 6.0 is recommended. Immunoprecipitation: 1:100 Western Blot: 1:1000-1:10,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Collagen Type II from human knee cartilage and bovine nasal cartilage.|
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Collagen Type II. Typically less than 1% crossreactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. Recognizes most mammalian Type II collagens and has expected reactivity with IV and VI. Negligible crossreactivity with Type I, III, V collagens. Non-specific crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested. Species Crossreactivity: bovine
