C7510-43H
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1,kClone Number
6F205 (1E7-D7/Col3)Grade
Affinity PurifiedApplications
E IHC WBCrossreactivity
Ca Hu RtGene ID
1281, 84032, 478835Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-Collagen Type III
Type III collagen is present in the walls of arteries, intestines and the uterus, where it strenghtens the connective tissue. Patients with defects in the type III collagen (ex. Ehlers-Danlos syndrome) are at risk of rupture of major arteries or the intestine. Can be used for detection of collagens by ELISA. Has been used successfully for immunohistology on frozen unfixed sections of human (3) and kangaroo (4) skin, and of new canine tissue associated with biomaterial implants (2,5). If fixation of tissue is required, acetone or ethanol is recommended.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: Unfixed, acetone or ethanol frozen sections. Western Blot: detects type III collagen only by its native triple helix form Optimal dilutions to be determined by the researcher.
Hybridoma
NS1/AG4.1 myeloma cells with spleen cells from mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Acid-digested pepsin soluble human type III collagen. Epitope: Located ~100nm from the N-terminal of the molecule.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
Purity
Purified by Protein A affinity chromatography from culture supernatant.
Specificity
Recognizes human type III collagen. Species Crossreactivity: canine, rat and kangaroo type III collagens by ELISA and Western Blot. The antibody does not show reactivity with calf and sheep type III collagen by ELISA and Western Blot. It has been shown to have no crossreactivity with type I and V collagens by ELISA and Western Blot. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
