Technical Data

C7510-50A
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b
Clone Number
1042
Grade
Supernatant
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Basement Membrane Alpha 2 Chain, Collagen Type IV Alpha 1, Collagen Type IV Alpha 2, DKFZp686I14213, FLJ22259, Goodpasture antigen)

The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antibody is important in the differential diagnosis of bullous lesions in dermatopathology.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (paraffin): 1:10-1:20; protease (pepsin) treatment and/or microwave treatment recommended Immunohistochemistry (frozen): 1:10-1:20 Optimal dilutions to be determined by the researcher.
Positive Control
Skin, kidney.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human placental type IV collagen.
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Supernatant
Specificity
Recognizes human collagen type IV. No crossreactivity with other collagens.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. M.G. Havenitz, J.P.M. Cleutjens, C. Beek, E.v.d.Linden, A.F.P.M. De Goeij, and F.T. Bosman: Human specific anti-type IV collagen monoclonal antibodies, characterization and immunohistochemical applicatiuon. Histochemistry (1987) 87:123-128.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net