Rabbit Anti-Collagen Type VI, alpha 1 (COL6a1, OPLL) (Biotin)
The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains. Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Collagen Type VI from human and bovine placenta.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (Immunoglobulin and Protease Free), 0.01% sodium azide. Reconstitute with 100ul sterile ddH2O. Labeled with Biotin.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Collagen Type VI. Reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Species Crossreactivity: bovine.
