Technical Data

C7850-14Y-ML650
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™650
Isotype
IgG1
Clone Number
053A-514.3.1.4
Grade
Affinity Purified
Applications
FC FLISA IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-Complement C3, C3d (Complement C3d Fragment, Complement Component C3d, C3/C3d, Complement Component 3, Complement Factor 3, CPAMD1) (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Human complement component 3d (C3d) neoantigen is a 33kD polypeptide fragment generated over the course of complement activation in which where complement 3 (C3) convertases cleave C3 to C3b, which is further degraded into iC3b and C3dg/C3d. C3d is involved in the regulation of many aspects of the immune response, including antigen processing and presentation. The C3d/antigen complex mediates B cell activation by simultaneously binding antigen-specific surface bound immunoglobulin and CD21, lowering the complement activation threshold. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
Applications
Suitable for use in FLISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Positive Control
Kidney from post streptococcal glomerulonephritius patients
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified human C3d (neoantigen).
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human complement component 3d (C3d) neoantigen at ~33kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bergmann­Leitner, E. et al. (2006) Complement 3d: From molecular adjuvant to target of immune escape mechanisms. Clinical Immunology. 121: 177­185. 2. Barilla­LaBarca, M. et al. (2002) Role of membrane cofactor protein (CD46) in regulation of C4b and C3b deposited on cells. J. Immunol. 168: 6298­6304.
USBio References
No references available
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