Goat Anti-Complement C4, Human (Not for Export EU)
C4 consists of three polypeptide chains (alpha, molecular weight 95kD, beta, molecular weight 75kD and gamma, molecular weight 33kD) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human C4 protein
Form
Supplied as a liquid in TBS, pH 7.5, 0.09% sodium azide
Specificity
Recognizes human complement component C4 (pooled normal human plasma and pooled 2X concentrated human serum) by immunoelectrophoresis
