Technical Data

C7850-51D-FITC
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG2b
Clone Number
10B403 (002-94.8.8)
Grade
Affinity Purified
Applications
FLISA
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free
Mouse Anti-Complement C9 (Complement Component 9, |Complement Component C9a, Complement Component C9b) (FITC)

Complement component 9 (C9) is a 71kD member of the complement C6/C7/C8/C9 family present in the blood serum and synthesized by the liver and monocytes. C9 is the last component to be added during the formation of the membrane attack complex (MAC), binding the membrane associated C5b-8 complex and resulting in the circular polymerization of 12-18 C9 molecules. This forms the hydrophilic transmembrane channel which causes cell lysis. Deficiency of C9 is associated with recurring infections by Neisseria meningitides.

Applications
Suitable for use in FLISA. Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified human Complement C9.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Complement C9. Does not recognize MAC.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Huang, Y. et al. (2006) Defining the CD59-C9 binding interaction. J Biol Chem. 281(37): 27398-404.
USBio References
No references available
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