Rabbit Anti-Connexin 29
Gap junctions have important functions in the transmission of signals across synapses in the nervous system (3). Connexin29 is a gap junction protein of 258 amino acids encoded by a novel single copy mouse gene that shows 75% sequence identity to human connexin 30.2 (4). Connexin29 mRNA (4.4 kb) is highly abundant in mouse sciatic nerve and is less abundant in spinal cord and adult brain (4). Expression data suggest that the new connexin gene is active in myelin-forming glial cells (4).
Applications
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Immunofluorescence: 0.5-2ug/ml Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Mouse brain or sciatic nerve homogenates
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide derived from the C-terminal sequence of mouse Cx29.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse Cx29. Two bands were observed in Western Blot; a band at ~29kD is the connexin29 monomer, a second band at ~58kD corresponds to the Cx29 dimer. Species Crossreactivity: human and rat.
