Technical Data

C7865-25C
Clone Type
Polyclonal
Host
Sheep
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Sheep Anti-Contactin 6 (Neural recognition molecule NB-3, hNB-3, Contactin-6 precursor, MGC133256, NB3, NB-3)

Contactin-6, is a 130kD member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1,2). Mature mouse Contactin-6 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3-5). Mouse Contactin-6 shares 90-96aa sequence identity with human and rat Contactin-6, respectively. Alternate splicing generates an isoform with a 17aa deletion in the first Ig-like domain (3). Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3,5-7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1 and in trans with Notch-1 on oligodendrocytes (6,8). Its binding to Notch-1 triggers translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5,7).

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa20-998 from mouse Contactin-6 expressed in CHO cells (Q9JMB8).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse Contactin-6. Species sequence homology: Recombinant mouse Contactin-3, Contactin-4, recombinant human Contactin-5, and TAG-1 (5%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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