Technical Data

C7905-01P-ML650
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™650
Isotype
IgG1
Clone Number
10B351 (C-PEP-01)
Grade
Purified
Applications
IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-C-Peptide (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

C-peptide also known as connecting peptide is a peptide released when proinsulin is cleaved to produce insulin.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human C-peptide conjugated to BSA.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human C-peptide. It can also be used to detect proinsulin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hilgert, I. et al. (1991) A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA.Hybridoma. 10: 379-86. 2. Afrikanova, I. et al. (2011) Inhibitors of Src and Focal Adhesion Kinase Promote Endocrine Specification: Impact on the Derivation of Beta Cells from Human Pluripotent Stem Cells. J Biol Chem. Aug 18. [Epub ahead of print] 3. Vasic, D. and Walcher, D. (2012) C-Peptide: A New Mediator of Atherosclerosis in DiabetesMediators of Inflammation. Article ID: 858692.
USBio References
No references available
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