Rat Anti-CRP (C-reactive Protein, PTX1)
CRP is a secreted protein that belongs to the pentraxin family. It circulates as a non-covalent homo-pentamer. CRP binds to several ligands and functions to initiate activation of the complement cascade, phagocytosis and inflammatory responses.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 2-8ug/ml Optimal dilutions to be determined by the researcher.
Hybridoma
Fusion of a mouse myeloma with B cells obtained from a mouse/rat immunized with purified, NS0-derived, recombinant mouse CRP.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Purified, NS0-derived, recombinant mouse CRP (aa1-225; Accession #P14847).
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse CRP.
