Rabbit Anti-CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein)
CREB is a b-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Chromatin Immunopreciptation. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Detected phosphorylated CREB (Ser133) in 10ug of forskolin treated BALB/3T3 cell lysate. . Immunoprecipitation: 5ug immunoprecipitated phospho CREB (Ser133) in 500ug of BALB/3T3 A31+Forskolin cell lysate. Immunohistochemistry: 1:50-1:1000 detects phosphorylated CREB in rat cerbral cortex, human cerebral cortex and rat hippocampus tissue. Chromatin Immunopreciptation: 4ug immunoprecipitates CREB (Ser133) in HEK293T cell lysate. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Linear peptide corresponding to the KID of human phospho CREB (Ser133), conjugated to KLH. Species Sequence Homology: bovine, canine, porcine, Xenopus, zebrafish.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4. 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CREB when phosphorylated on Ser133, Mr ~43kD. Species Crossreactivity: mouse and rat.
