Technical Data

C7915-12
Grade
Lysate
Shipping Temp
Blue Ice
Storage Temp
-20°C
Anti-CREB Control Cell Extracts (cAMP Response Element Binding Protein)

Transcription factor CREB binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinases CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity (1,2). Growth and neurotrophic factors also stimulate CREB phosphorylation at Ser133 (4). Phosphorylation occurs at Ser133 via p44/42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK1 (5–7). CREB appears to play an important role in learning and memory in both flies and mice. Mice lacking CREB exhibit deficiencies in spatial learning tasks, while flies overexpressing or lacking CREB show enhanced or diminished learning, respectively (8–10). Nonphosphorylated CREB Control Cell Extracts: Total cell extracts from SK-N-MC cells prepared without treatment serve as a negative control. Supplied in SDS Sample Buffer. Phosphorylated CREB Control Cell Extracts: Total cell extracts from SK-N-MC cells prepared with IBMX and forskolin treatment serve as a positive control. Supplied in SDS Sample Buffer.

Source
SK-N-MC cells
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in SDS Sample Buffer: 62.5mM Tris-HCl, pH 6.8 at 25°C, 2% w/v SDS, 10% glycerol, 50mM DTT, 0.01% w/v bromophenol blue.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
(1) Gonzalez, G.A. and Montminy, M.R. (1989) Cell 59, 675–680. (2) Sheng, M. et al. (1991) Science 252, 1427–1430. (3) Ginty, D.D. et al. (1994) Cell 77, 713–725. (4) Bourtchuladze, R. et al. (1994) Cell 79, 59–68. (5) Xing, J. et al. (1996) Science 273, 959–962. (6) Tan, Y. et al. (1996) EMBO J. 15, 4629–4642. (7) Deak, M. et al. (1998) EMBO J. 17, 4426–4441. (8) Frank, D.A. and Greenberg, M.E. (1994) Cell 79, 5–8. (9) Yin, J.C.P. et al. (1995) Cell 81, 107–115. (10) Yin, J.C.P. et al. (1994) Cell 79, 49–58.
USBio References
No references available
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