Technical Data

C7922-10H
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Cripto (CRGF, Cripto-1 Growth Factor, Cripto-3, TDGF1, TDGF2, Teratocarcinoma-derived growth factor 1)
CRGF, Cripto-1 Growth Factor, Cripto-3, TDGF1, TDGF2, Teratocarcinoma-derived growth factor 1||||

The cripto gene is expressed both in ES cells and during the early phases of embryo development, while, in the adult, it is reactivated in a wide range of epithelial cancers.

Cellular Localization: Membrane-bound
Positive Control: MDA-MB231 (human breast cancer) lysates
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A synthetic peptide made to an N-terminal portion of the murine cripto protein sequence (between residues 1-50).
Form
Tris-glycine and 150mM NaCl
Purity
Purified by peptide immunoaffinity chromatography.
Specificity
This antibody is specific for cripto. Species Crossreactivity: This antibody reacts with human cripto protein. The immunogen used for this antibody production has 78% homology with human protein and 100% with mouse protein. There is no significant homology with any other species.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Xian, PX, et al. Cancer Res. 64(11): 4018-23 (2004)|2. Niemeyer, CC, et al. Int. J. Cancer. 81(4): 588-91 (1999)|3. Adamson, E., et al. Cripto: A tumor growth factor and more. J. Cell. Physiol. 190(3):2567-278, 2002.|4. Schiffer, S.G., et al. Fucosylation of cripto is required for its ability to facilitate nodal signaling. J. Biol. Chem. 276(41):37769-37778, 2001.
USBio References
No references available
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