Technical Data

C7925-01
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_005198
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-CrkL (Crk-like protein, v-crk sarcoma virus CT10 oncogene homolog (avian)-like)

CrkL is a 39kD adaptor protein, originally cloned in proximity to the BCR gene on chromosome 22, which has a key regulatory role in hematopoietic cells. CrkL has one SH2 and two SH3 domains, with 60% homology to CrkII (1). Structurally, the amino-terminal SH3 domain of CrkL has been shown to bind proteins such as C3G, SOS, PI3K, c-Abl or BCR/Abl. The SH2 domain of CrkL can bind to tyrosine-phosphorylated proteins such as Cbl, HEF1, CAS or paxillin (2,3). CrkL is involved in various signalings initiated by different cytokines and growth factors. The biological outcomes of the Crk-activated signal transduction cascades include the modulation of cell adhesion, cell migration, immune cell responses and so on (4). CrkL is a prominent substrate of the BCR/Abl oncoprotein in chronic myelogenous leukemia and binds to both BCR/Abl and c-Abl (5). CrkL is prominently and constitutively tyrosine phosphorylated in CML neutrophils and is not phosphorylated in normal neutrophils. Moreover, stimulation of normal neutrophils with cytokines and agonists does not induce tyrosine phosphorylation of this protein (6), indicating that it may be a useful target for therapeutic intervention or as a disease marker. Tyrosine 207 in CrkL is the BCR/Abl phosphorylation site (7).

Applications
Suitable for use Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.01-0.03ug/ml. Detects a band of ~37kD in lysates of cell lines K562 (calculated MW of 33.8kD according to NP_005198.1) Peptide ELISA: 1:16,000 Immunohistochemistry (paraffin): 2.5-3.8ug/ml, in paraffin embedded Human Thymus shows staining of cellular membranes in thymocytes. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-KIFDPQNPDENE from the C-terminus of human CRKL. Species sequence homology: mouse and rat
Form
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CRKL.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Satter, M., Salgia, R., Leukemia 12: 637-644 (1998). 2. Feller, S.M., et al., J. Cell. Physiol. 177: 535-552 (1998). 3. Kiyokawa, E., et al., Crit. Rev. Oncog. 8: 329-342 (1997). 4. Feller, S.M., et al., Oncogene 20: 6348-6371 (2001). 5. Grumbach, I.M., et al., Br. J. Haematol. 112: 327-336 (2001). 6. Nicholas, G.L., et al., Blood 84: 2912–291 (1994). 7. de Jong, R., et al., Oncogene 14: 507-513 (1997).
USBio References
No references available
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