Goat Anti-Crystallin AB (Alpha crystallin B chain, CryAB, Alpha (B)-crystallin, Rosenthal fiber component, Heat-shock protein beta-5, HspB5, CryA2, AACRYA, Alpha crystallin B chain, CRYA2, Crystallin alpha polypeptide 2, CTPP 2, CTPP2, Heat shock 20kD like protein, Heat shock protein beta 5, HSPB5, Rosenthal fiber component)
AlphaB Crystallin (CRYAB, also known as Crystallin alpha-B chain and HspB5) is a 22-23kD member of the HSP20 family of proteins. It has widespread expression in many tissues in addition to lens epithelium, where it noncovalently oligomerizes with CRY-alphaA to generate a transparent 350-1000kD alpha-Crystallin protein complex. Human CRYAB is 175aa in length. There is a alpha-Crystallin Hsp domain over aa66-149. Multiple posttranslational modifications may exist. The N-terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved. There is also phosphorylation at Ser45 and 59, potential O-GlcNAc modification at Thr158, 162 or 170, and acetylation at Lys92. There is an alternate start site at Met68 that may be accompanied by a 47aa substitution for aa109-175. Full-length human CRYAB is 54% aa identical to CRY-AlphaA, and 98% aa identical to mouse CRYAB.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 0.1ug/ml Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-28C: IgG, H&L (HRP) Pab DkxGt I1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab DkxGt I1904-28K: IgG, H&L (HRP) Pab RbxGt I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-derived recombinant human Crystallin AB (aa1-175, Accession #P02511)
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 100ul sterile PBS.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human Crystallin AB (CRYAB) at ~23kD. Does not crossreact with recombinant human CRYAA in Western Blots. Species Crossreactivity: mouse and rat
