C7945-50F
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Bo Ca Hu Mo RtAccession #
NP_997657.1, NP_004118.3Gene ID
2873, 209318, 117039Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-CSN1 (COP9 Signalosome Complex Subunit 1, SGN1, Signalosome Subunit 1, G-Protein Pathway Suppressor 1, GPS-1, JAB1-containing Signalosome Subunit 1, Protein MFH, GPS1, COPS1)
Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein- and mitogen-activated protein kinase-mediated signal transduction.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Western Blot: 0.5ug/ml, observed in human testis lysate on ~55kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-PANSQSRMSTNM, from human GPS1, at C-terminal (NP_997657.1, NP_004118.3).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human GPS1. Species sequence homology: Bovine, canine, mouse and rat.
